Abstract 12: Fibromodulin Selectively Promotes Myofibroblast Apoptosis During Cutaneous Wound Healing
نویسندگان
چکیده
METHODS: A resected VM specimen was digested and cells sorted with CD133+ magnetic beads. Isolated cells were analyzed by FACS with antibodies against endothelial progenitor markers, inflammatory cell markers, and differentiated EC markers. Cells were re-suspended in Matrigel and injected into an immunocompromised mouse. After 3 weeks, implants were harvested and stained with an antibody against ECs marker, CD31.
منابع مشابه
Negative Regulation of GADD34 on Myofibroblasts during Cutaneous Wound Healing
The growth arrest and DNA damage-inducible protein, GADD34, has been proved to be involved in TGF-β signaling pathway and correlates with cell death, which are two important mechanisms in regulating myofibroblast differentiation and apoptosis during tissue repair. But roles of GADD34 in myofibroblasts differentiation and apoptosis remain unknown. To investigate the function of GADD34 in these p...
متن کاملFibromodulin Enhances Angiogenesis during Cutaneous Wound Healing
BACKGROUND Fibromodulin (FMOD) plays a critical role in the wound-healing process. Our previous studies revealed that FMOD deficiency led to marked alterations in adult wound healing characterized by delayed dermal cell migration, postponed wound closure, and increased scar formation, all accompanied by impeded angiogenesis. Therefore, the aim of this study was to reveal the effect of FMOD on a...
متن کاملDiminished type III collagen promotes myofibroblast differentiation and increases scar deposition in cutaneous wound healing.
The repair of cutaneous wounds in the postnatal animal is associated with the development of scar tissue. Directing cell activities to efficiently heal wounds while minimizing the development of scar tissue is a major goal of wound management and the focus of intensive research efforts. Type III collagen (Col3), expressed in early granulation tissue, has been proposed to play a prominent role i...
متن کاملTGF-ß1 Latency Associated Peptide Promotes Remodeling of Healing Cutaneous Wounds in the Rat
Background: The process of wound healing involves integrated events including inflammation, granulation tissue formation, matrix deposition and remodeling. Growth factors play a key role in the process. Among them transforming growth factor-ß1 (TGF-ß1) is known to accelerate tissue repair by promoting the synthesis and deposition of extracellular matrix proteins. However, persistence or overact...
متن کاملFormation and function of the myofibroblast during tissue repair.
It is generally accepted that fibroblast-to-myofibroblast differentiation represents a key event during wound healing and tissue repair. The high contractile force generated by myofibroblasts is beneficial for physiological tissue remodeling but detrimental for tissue function when it becomes excessive such as in hypertrophic scars, in virtually all fibrotic diseases and during stroma reaction ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره 5 شماره
صفحات -
تاریخ انتشار 2017